Ultrastructural, opto-physiological and behavioral analysis of synaptic organization within the aging Drosophila olfactory system.

Ultrastructural, opto-physiological and behavioral analysis of synaptic organization within the aging Drosophila olfactory
system. (a-d) Adult brains of 3d and 30d flies expressing 4xBRP (c-d) together with age-matched 2xbrp controls in (a-b) immunostained for BRP (using Nc82 and BRP N-terminal antibody) and RBP (Rim-Binding-Protein, another active zone scaffold protein(Liu et al.,2011)). Scale bar: 50 μm. (e, f) Quantification of BRP (using N-terminal antibody) as well as RBP intensity within the central brain region normalized to 3d flies (n = 12±13 independent brains; (g-j) STED images of BRP label within the calyx region of 3d and 30d flies expressing 4xBRP as well as 2xBRP. Ring-shaped structures are indicated (arrowheads). Scale bar: 500 nm. (k) Quantification of BRP ring diameter in 3d and 30d 4xBRP flies along with age-matched 2xBRP flies (total of 47±68 BRP rings across eight independent animals, with at least six BRP rings per animal; Kruskal-Wallis test with Dunn’s multiple comparison test, p-values were subject to Bonferroni correction). Data taken from (Gupta et al., 2016).